Personal profile in our Scientific Output Portal (PPC)  

UNIVERSITY EDUCATION

1990: PhD in Biology (Virology), Faculty of Science, University Autónoma , Madrid , Spain . Title: "Genetic and phenotypic variation during a persistent infection of the foot-and mouth disease virus in cell culture".

1985: B.S. in Biology (Biochemistry and Molecular Biology), Faculty of Biology, University of Sevilla , Spain .

1980-83: First cycle of Biology, Faculty of Biology, University of Murcia , Spain .

Research lines

Role of host factors in the replication of positive strand RNA viruses.

Publications

• Pérez-Vilaró G, Jungfleisch J, Saludes V, Scheller N, Giménez-Barcons M, Díez J. Host factors in viral life cycles. Math Model Nat Phenom., in press.
• Pérez-Vilaró G, Scheller N, Saludes V, Díez J. Hepatitis C virus alters P-body composition but is independent of P-body granules. J Virol. 2012 Aug;86(16):8740-9.
• Diez J, Martinez JP, Mestres J, Sasse F, Frank R, Meyerhans A. Myxobacteria: natural pharmaceutical factories. Microb Cell Fact. 2012 Apr 30;11:52. 
• Giménez-Barcons M, Díez J. Yeast processing bodies and stress granules: self-assembly ribonucleoprotein particles.Microb Cell Fact. 2011 Sep 24;10:73. 
• Ehrhardt M, Leidinger P, Keller A, Baumert T, Díez J, Meese E, Meyerhans A. Profund differences of microRNA expression patterns in hepatocytes and hepatoma cell lines commonly used in hepatitis C virus studies. Hepatology. 2011 Sep 2;54(3):1112-3.  
• Reiter J, Pérez-Vilaró G, Scheller N, Mina LB, Díez J., Meyerhans A. Hepatitis C recombination in cell culture. J Hepatol. 2011 Oct;55(4):777-83. 
• Galão RP, Chari A, Alves-Rodrigues I, Lobão D, Mas A, Kambach C, Fischer U, Díez J. LSm1-7 complexes bind to specific sites in viral RNA genomes and regulate their translation and replication. RNA. 2010 Apr;16(4):817-27.
• Scheller N, Díez J. RNA viruses hijack the mRNA decay machinery to multiply. Cell Cycle. Dec 2009 8:24.4013-4014.  
• Scheller N, Mina LB, Galão RP, Chari A, Giménez-Barcons M, Noueiry A, Fischer U, Meyerhans A, Díez J. Translation and replication of hepatitis C virus genomic RNA depends on ancient cellular proteins that control mRNA fates. Proc Natl Acad Sci U S A. Aug 2009;106:32.13517-13522 .
• Scheller N, Resa-Infante P, de la Luna S, Galao RP, Albrecht M, Kaestner L, Lipp P, Lengauer T, Meyerhans A, Díez J. 
• Biochim Biophys Acta. 2007 Dec; 1773 ( 12 ): 1786-92.
• Galao R., Schelller, N.,  Alves-Rodrigues, I., Breinig, T., Meyerhans, A., Díez, J. 2007. Saccharomyces cerevisiae: a versatile eukaryotic system in virology. Microb. Cell Fact. 6: 32
• Scheller, N., Resa-Infante, P., de la Luna, S., Galao R. P., Albrecht, M., Kaestner, L., Lipps, P., Lengauer, T., Meyerhans, A., Díez, J. Identification of PatL1, a human homolog to yeast P body component Pat1. BBA Mol Cell Res.1773(12).1786-92.                                                                                                    
• Alves-Rodrigues, I., Mas, A., Díez, J. 2007. Xenopus Xp54 and human RCK/p54 helicases functionally replace yeast Dhh1p in Brome Mosaic Virus RNA replication . J. Virol. 81(8): 4378-4380.
• Alves-Rodrigues, I., Galao R., Meyerhans, A., Díez, J. 2006. Saccharomyces cerevisiae:  auseful model to study fundamental biology of viral replication. Virus Res. 120: 49-56.                                   
• Mas, A., Alves-Rodríguez, I., Noueiry, A., Ahlquist, P., Díez, J. 2006. Host deadenylation-dependent mRNA decapping factors are required for a key step in brome mosaic virus RNA replication.. J. Virol. 80(1): 246-251.       
• Díez, J. 2006. Virus de la Hepatitis C . In : Virus  Patógenos. Ed Hélice. 365-375.
• Solé, R.V., Sardanyés, J., Díez, J. and Mas, A. 2006. Information catastrophe in RNA viruses through replication thresholds: J. Theor. Biol.240(3):353-9.                                                                   
• Mas. A., Ulloa, E., Bruguera, M., Farcic, I.,  Garriga, D., Fábregas, S., Andreu, D., Saíz, JC, Díez, J. 2004. Hepatitis C virus population analysis of a single source nosocomial outbreak reveals an inverse correlation between viral load and quasispecies complexity. J. Gen. Virol. 85: 3619-3626.
• Garriga, D., Díez, J., Oliva, B. 2004. Modeling of the helicase domain of Brome Mosaic Virus 1a replicase. J. Mol. Model. 5-6: 382-392.
• Noueiry, A., Díez, J., Falk, S.P., Chen, J., Ahlquist, P. 2003. Yeast Lsm1p-7p/Pat1p deadenylation-dependent mRNA decapping factors are required for brome mosaic virus RNA translation . Mol. Cell Biol. 23 (12): 4094-4106.
• Tomita, Y., Mizuno, T., Díez, J., Satoshi, N., Ahlquist, P., Isikawa, M. 2003. Mutation of host dnaJ homolog inhibits negative-strand RNA synthesis of brome mosaic virus. J. Virol. 77(5): 2990-2997
• Díez, J., Isikawa, M., Kaido, M., Ahlquist, P. 2000. Identification and Characterization of a host protein involved in template selection for viral RNA replication. Proc. Natl. Acad Sci. USA. 97 (8): 3913-3918.
• Díez, J., Marcos, J.F., Pallás, V. 1999. Characterization  and in vitro translation of Pelargonium Flower Brake Virus. Arch. Virol. 144(8): 1627-1637.
• Pallas, V., Sanchez-Navarro, J.A., Díez, J. 1999. In vitro evidence of RNA binding properties of the coat protein of prunus necrotic ringspot ilarirus and their comparaison to related and unrelated viruses. Arch. Virol. 144 (4): 797-803
• Díez, J., Marcos, J.F., Pallás, V. 1998. Carmovirus isolation and RNA extraction. Methods Mol Biol. 81; 211-217.                                                                                                                                  
• Isikawa, M., Díez, J., Restrepo-Hartwig, Ahlquist, P. 1997. Yeast mutations in multiple complementation groups inhibit brome mosaic virus RNA replication and trasncriprion and perturb regulated expression of the viral polymerase-like gene.Proc. Natl. Acad Sci. USA. 94 (25): 13810 -13815.
• Ahlquist, P., Díez, J., Isikawa, M., Janda, M., Noueiry, A., Price, B.D., Restrepo-Hartwig, M. Sullivan, M. 1996. Interactive Role of Viral Proteins, Viral RNA, and Host Factors in Bromovirus RNA Replication. In: Biology of Plant-Microbe Interactions (G. Stacy, B. Mullin and P. Gresshoff, eds.) pp. 281-286.
• Ahlquist, P., Isikawa, M., Díez, J., Noueiry, A, Janda, M., Price, B.D. 1996. Yeast as a model host to study viral replication mechanism and virus-host interaction. Proceeding of the First Shizuoka Forum on Health and Longevity pp 154-156
• Pallas, V., Sanchez-Navarro, J.A.,  Díez, J., Rowhani, A., Desvignes, J.C., Sanchez-Pina, M.A. 1995. Characterization and Genomic organization of Apple Mosaic Ilarvirus. Acta Horticulturae 386:100-108
• Domingo, E., Díez, J., Martínez, M.A., Hernández, J., Borrego, B., Mateu, M.G. 1993. New observation on antigenic diversification of RNA viruses. Antigenic variation is not dependent of immune selection. J. Gen. Virol. 74: 2034-2045.
• Díez, J., Dávila, M., Escarmís, C., Mateu, M.G. Dominguez, J. Pérez, J.J., Giralt. E., Melero, J.A., Domingo, E. 1991. Unique amino acid substitutions in the capsid proteins of foot-and-mouth disease virus from persistent infection in cell culture. J. Virol. 64 (11): 5519-5528.
• Domingo, E., Díez, J., Villaverde, A., Mateu, M.G. 1990. Paths of RNA virus evolution in human and animal diseases. Mol. Gen. (Life. Sci. Adv.) 9: 23-29.
• Diez, J., Mateu, M.G., Domingo, E. 1990. An in situ Elisa with FMDV plaques. In : Food and Agriculture Organization of the United States. Lindholm. Denmark.
• Díez, J., Hoffner, M., Domingo, E., Donaldson, A. 1990. Foot-and-mouth disease virus strains isolated from a persistently infected cell culture are attenuated for mice and cattle. Virus Res. 18 (11): 3-8
• Domingo, E., Díez, J., Mateu, M.G., Andreu, D., Giralt, E. 1990. RNA virus variation and vaccine design. IN " From genes to bioproducts". Ed. DM-PPU. pp 243-248.
• Diez, J., Mateu, M.G., Domingo, E. 1989. Selection of antigenic variants of foot-and-mouth disease virus in the absence of antibodies, as revealed by an in situ assay. J. Gen. Virol. 70: 3281-3289.
• de la Torre, J.C., Martínez-Salas, E., Diez, J., Domingo, E. 1989. Extensive cell heterogeneity during a persistent infection with foot-and-mouth disease virus. J. Virol. 63 (1): 59-63.
• de la Torre, J.C., de la Luna, S., Diez, J., Domingo, E. 1989. Resistence to foot-and-mouth disease virus mediated by trans-acting cellular product. J. Virol. 63(5): 2385-2387.
• de la Torre, J.C., Martínez-Salas, E., Diez, J., Villaverde, A., Gebauer, F. Rocha, E. Dávila, M., Domingo, E. 1988. Coevolution of cells and viruses in a persistent infection of foot-and-mouth disease virus in cell culture. J. Virol. 62(6): 2050-2058.