Pioneering technique to discover viruses present in saliva
Pioneering technique to discover viruses present in saliva
Scientists from University of Alicante and Pompeu Fabra University have used a recently developed technique to obtain genetic information from the viral community of human saliva. By using this technique, they have managed to sequence the viruses' DNA by separating them one by one. The work has been published in the journal Viruses.
A group of researchers led by Manuel Martínez García, member of the University of Alicante Department of Physiology, Genetics and Microbiology, has discovered new viruses present in human saliva through the application of techniques that combine flow cytometry, genomics and molecular biology. The work, published in the journal Viruses, has been participated by Òscar Fornas, researcher and head of the Pompeu Fabra University (UPF) and the Centre for Genomic Regulation (CRG) Flow Cytometry Unit.
The technique they have used is called single-virus genomics (SVGs) and consists of separating a single virus by flow cytometry, breaking its capsid, making copies of the genome and sequencing its DNA to be able to identify it.
Researchers have applied this technique to saliva samples of 15 volunteers and have managed to isolate 1,300 viruses and amplify the genome DNA in around 200 viruses. This way they have discovered eight new viruses, which are predominant members in the viral communities of the samples analysed. Results suggest a variable, complex and different virus profile in each person.
Researcher Manuel Martínez, currently in an advanced stage of the project, clarifies that "we are focusing on comparing the changes that occur in these viruses, and also bacteria, present in the saliva of healthy and sick people with certain underlying diseases such as various immunodeficiencies".
"The study is an example of the potential of this new technique to reveal genetic diversity of viruses in the human body since it could be implemented with any liquid sample," Oscar Fornas concluded.
The use of SVGs, in the study “Single-virus genomics reveals hidden cosmopolitan and abundant viruses” published in Nature Communications in 2017, allowed scientists to discover abundant marine viruses impossible to grow in the laboratory because of the technical difficulties involved. After demonstrating that this technique allowed them to study the ecology of groups of viruses in aquatic samples, they made the decision to address the viruses present in different ecosystems, such as the human body.
Reference article:
Cruz MJ, Martinez-Hernandez F, Garcia-Heredia I, Lluesma M, Fornas O and Martinez-Garcia M. Deciphering the Human Virome with Single-Virus Genomics and Metagenomics. Viruses, March 2018.