Flow Cytometry Unit is a UPF/CRG joint Facility and provides to PRBB researchers technical expertise and training to access the state-of-the-art instrumentation, as well as technical and scientific advice to develop efficient and reliable flow cytometric assays with the highest quality control standards and productivity.
Flow cytometry studies optical parameters emitted by particles (cells, cell fractions). Flow cytometers can study a series of parameters of individual particles simultaneously, quickly and on a large number of individualized particles in suspension. Flow cytometers use lasers as a source of light excitement; therefore, it must be possible to mark the particles with one or more fluorescent substances. Information is also collected regarding the size and structural complexity of each particle. This multiparameter study of each particle enables us to analyse subpopulations in complex samples through electronic sorting. Some cytometers are also equipped with sorting and collection modules for particles of interest.
The Unit currently hosts seven analyzers and two cell sorters supporting the use of a wide range of flow cytometry applications and new ones are developed/implemented responding to the facility needs or upon user demand.
New technology acquisition:
New Aurora (Cytek) has been acquired to cover advanced multicolour flow cytometry assays. This new technology facilitates the access to complex phenotype analysis while managing up to 48 fluorescences at the same time. Also simplifies the access to complex multicolor panel analyzing the full spectra of every cell.
Flow Karyotyping for chromosome sorting was implemented in our facility to isolate chromosomes for chromosome sequencing. Additionally, this methodology allows the detection of numerical and structural chromosome aberrations. Chromosomes of a single type can be purified facilitating gene mapping or production of chromosome-specific recombinant DNA libraries.
Single Virus Sorting has been developed in our facility to isolate single virus. Single virus isolation allows the access to genome of single virus for sequencing. Single Virus Genomics enable the discovery of some of the likely most abundant and ecologically relevant marine viral species. The method could be used to study other biological entities into different ecosystems.
Pioneering technique to discover viruses present in saliva (22/03/2018)
A group of researchers led by Manuel Martínez García, member of the University of Alicante Department of Physiology, Genetics and Microbiology, with the participation of Oscar Fornas, unit leader at the UPF-CRG Flow Cytometry Unit, has discovered new viruses present in human saliva through the application of techniques that combine flow cytometry, genomics and molecular biology.
Cruz MJ, Martinez-Hernandez F, Garcia-Heredia I, Lluesma M, Fornas O and Martinez-Garcia M.
"Deciphering the Human Virome with Single-Virus Genomics and Metagenomics."
Viruses, March 2018.
- Vara C, Paytuví-Gallart A, Cuartero Y, Le Dily F, Garcia F, Salvà-Castro J, Gómez-H L, Julià E, Moutinho C, Aiese Cigliano R, Sanseverino W, Fornas O, Pendás AM, Heyn H, Waters PD, Marti-Renom MA, Ruiz-Herrera A. T Cell Rep. 2019 Jul 9;28(2):352-367.e9
- Selective single molecule sequencing and assembly of a human Y chromosome of African origin.Kuderna LFK, Lizano E, Julià E, Gomez-Garrido J, Serres-Armero A, Kuhlwilm M, Alandes RA, Alvarez-Estape M, Juan D, Simon H, Alioto T, Gut M, Gut I, Schierup MH, Fornas O, Marques-Bonet T. Nat Commun. 2019 Jan 2;10(1):4.
- Single-cell genomics uncover Pelagibacter as the putative host of the extremely abundant uncultured 37-F6 viral population in the ocean. Martinez-Hernandez F, Fornas Ò, Lluesma Gomez M, Garcia-Heredia I, Maestre-Carballa L, López-Pérez M, Haro-Moreno JM, Rodriguez-Valera F, Martinez-Garcia M. ISME J. 2019 Jan;13(1):232-236.
- Deciphering the Human Virome with Single-Virus Genomics and Metagenomics. de la Cruz Peña MJ, Martinez-Hernandez F, Garcia-Heredia I, Lluesma Gomez M, Fornas Ò, Martinez-Garcia M. Viruses. 2018 Mar 6;10(3). pii: E113.
- Single-virus genomics reveals hidden cosmopolitan and abundant viruses. Martinez-Hernandez F, Fornas O, Lluesma Gomez M, Bolduc B, de la Cruz Peña MJ, Martínez JM, Anton J, Gasol JM, Rosselli R, Rodriguez-Valera F, Sullivan MB, Acinas SG, Martinez-Garcia M. Nat Commun. 2017 Jun 23;8:15892.
- Triplication of DYRK1A causes retinal structural and functional alterations in Down syndrome. Laguna A, Barallobre MJ, Marchena MÁ, Mateus C, Ramírez E, Martínez-Cue C, Delabar JM, Castelo-Branco M, de la Villa P, Arbonés ML. Hum Mol Genet. 2013 Jul 15;22(14):2775-84. doi: 10.1093/hmg/ddt125. Epub 2013 Mar 19.