Boronat, S., Cabrera, M. and Hidalgo, E. (2021) Spatial sequestration of misfolded proteins as an active chaperone-mediated process during heat stress. Curr. Genet. (in press).



Boronat, S., Marte, L., Vega, M., García-Santamarina, S., Cabrera, M., Ayté, J. and Hidalgo, E. (2020) The Hsp40 Mas5 connects protein quality control and the general stress response through the thermo-sensitive Pyp1.  iScience 23: 101725.

Sánchez-Mir, L., Fraile, R., Ayté, J. and Hidalgo, E. (2020) Phosphorylation of the transcription factor Atf1 at multiple sites by the MAP kinase Sty1 controls homologous recombination and transcription.  J. Mol. Biol. 432:5430-5446.

Sansó, M., Parua, P.K., Pinto, D., Svensson, J.P., Pagé, V., Bitton, D.A., MacKinnon, S., García, P., Hidalgo, E., Bähler, J., Tanny, J.C. and Fisher, R.P. (2020)  Cdk9 and H2Bub1 signal to Clr6-CII/Rpd3S to suppress aberrant antisense transcription.  Nucleic Acids Res. 48:7154-7168.

Fraile, R., Sánchez-Mir, L. and Hidalgo, E. (2020) A new adaptation strategy to glucose starvation: modulation of the gluconate shunt and pentose phosphate pathway by the transcriptional repressor Rsv1.  FEBS J. 287:874-877.

Cabrera, M., Boronat, S., Marte, L., Vega, M., Pérez, P., Ayté, J. and Hidalgo, E. (2020) Chaperone-facilitated aggregation of thermo-sensitive proteins shields them from degradation during heat stress.  Cell Rep. 30:2430-2443.

Marte, L., Boronat, S., García-Santamarina, S., Ayté, J., Kitamura, K. and Hidalgo, E. (2020) Identification of ubiquitin-proteasome system components affecting the degradation of the transcription factor Pap1.  Redox Biol. 28:101305.



Henríquez-Olguín C., Boronat, S., Cabello-Verrugio, C., Jaimovich, E., Hidalgo, E. and Jensen, T.E. (2019) Emerging role of NADPH oxidase 2 in skeletal muscle redox signaling and metabolism. Antioxid. Redox Signal. 31:1371-1410.

Carmona, M., de Cubas, L., Bautista, E., Moral-Blanch, M., Medrano-Fernández, I., Sitia, R., Boronat, S., Ayté, J. and Hidalgo, E. (2019) Monitoring cytosolic H2O2 fluctuations arising from altered plasma membrane gradients or from mitochondrial activity. Nat. Commun. 10:4526.

González-Medina, A., Hidalgo, E. and Ayté, J. (2019) Gcn5-mediated acetylation at MBF-regulated promoters induces the G1/S transcriptional wave. Nucleic Acids Res. 47:8439-8451.



Knezevic, I., González-Medina, A., Gaspa, L., Hidalgo, E. and Ayté, J. (2018) The INO80 complex activates the transcription of S phase genes in a cell cycle regulated manner. FEBS J. 285:3870-3881.

Domènech, A., Ayté, J., Antunes, F. and Hidalgo, E. (2018). Using in vivo oxidation status of one- and two-component redox relays to determine H2O2 levels linked to signaling and toxicity. BMC Biol. 16:61.

Sánchez-Mir, L., Salat-Canela, C., Paulo, E., Carmona, M., Ayté, J., Oliva, B. and Hidalgo E. (2018). Phospho-mimicking Atf1 mutants bypass the transcription activating function of the MAP kinase Sty1 of fission yeast. Curr. Genet. 64:97-102.



Salat-Canela, C., Paulo, E., Sánchez-Mir, L., Carmona, M., Ayté, J., Oliva, B. and Hidalgo E. (2017). Deciphering the role of the signal- and Sty1 kinase-dependent phosphorylation of the stress-responsive transcription factor Atf1 on gene activation. J. Biol. Chem. 292:13635-13644.

Boronat, S., Domènech, A., Carmona, M., García-Santamarina, S., Bañó, M.C., Ayté, J. and Hidalgo, E. (2017). Lack of a peroxiredoxin suppresses the lethality of cells devoid of electron donors by channelling electrons to oxidized ribonucleotide reductase.  PLoS Genet. 13(6):e1006858.

Boronat, S., Domènech, A. and Hidalgo, E. (2017) Proteomic characterization of reversible thiol oxidations in proteomes and proteins. Antioxid. Redox Signal. 26:329-344.



Shah, M., Su, D., Scheliga, J.S., Pluskal, T., Boronat, S., Motamedchaboki, K., Rosa Campos, A., Qi, F., Hidalgo, E., Yanagida, M. and Wolf, D.A. (2016) A Transcript-Specific eIF3 Complex Mediates Global Translational Control of Energy Metabolism. Cell Reports 16:1891-902.

Gaspa, L., González-Medina, A., Hidalgo, E. and Ayté, J. (2016) A functional genome-wide genetic screening identifies new pathways controlling the G1/S transcriptional wave. Cell Cycle 15:720-72.

Alves-Rodrigues, I., Ferreira, P.G., Moldón, A., Vivancos, A.P., Hidalgo, E., Guigó, R and Ayté, J. (2016) Spatiotemporal control of forkheads binding to DNA regulates the meiotic gene expression program. Cell Reports 14:885-895.

Eckert, D., Andrée, N., Razanau, A., Zock-Emmenthal, S., Lützelberger, M., Plath, S., Schmidt, H., Guerra-Moreno, A., Cozzuto, L., Ayté, J. and Käufer, N.F. (2016) Prp4 kinase grants the license to splice: control of weak splice sites during spliceosome activation. PLoS Genet. 12(1):e1005768.

García, P., Encinar del Dedo, J., Ayté, J. and Hidalgo, E. (2016) Genome-wide Screening of Regulators of Catalase Expression: Role of a Transcription Complex and Histone and tRNA Modification Complexes on Adaptation to Stress. J. Biol. Chem. 291:790-799.



Vázquez, B., Soto, T., Del Dedo, J.E., Franco, A., Vicente, J., Hidalgo, E., Gacto, M., Cansado, J. and Madrid, M. (2015) Distinct biological activity of threonine monophosphorylated MAPK isoforms during the stress response in fission yeast. Cell Signal. 27:2534-2542.

Materne, P., Anandhakumar, J., Migeot, V., Soriano, I., Yague-Sanz, C., Hidalgo, E., Mignion, C., Quintales, L., Antequera, F. and Hermand, D. (2015) Promoter nucleosome dynamics regulated by signaling through the CTD code.  eLife4:e09008.

Encinar del Dedo, J., Gabrielli, N., Carmona, M., Ayté, J., and Hidalgo, E. (2015) A cascade of iron-containing proteins governs the genetic iron starvation response to promote iron uptake and inhibit iron storage in fission yeast. PLoS Genet. 11(3):e1005106.

Boronat, S., García-Santamarina, S. and Hidalgo, E.  (2015) Gel-free proteomic methodologies to study reversible cysteine oxidation and irreversible protein carbonyl formation.  Free Radic. Res. 49:494-510.



García, P., Paulo, E., Gao, J., Wahls, W., Ayté, J., Lowy, E. and Hidalgo, E. (2014) Binding of the transcription factor Atf1 to promoters serves as a barrier to phase nucleosome arrays and avoid cryptic transcription. Nucleic Acids Res. 42:10351-10359.

García-Santamarina, S., Boronat, S. and Hidalgo, E. (2014) Reversible cysteine oxidation in hydrogen peroxide sensing and signal transduction. Biochemistry 53:2560:2580.

Paulo, E., García-Santamarina, S., Calvo, I.A., Carmona, M., Boronat, S., Domènech, A., Ayté, J. and Hidalgo, E. (2014) A genetic approach to study H2O2 scavenging in fission yeast - distinct roles of peroxiredoxin and catalase.  Mol. Microbiol. 92:246-257.

García-Santamarina, S., Boronat, S., Domènech, A., Ayté, J., Molina, H. and Hidalgo, E. (2014) Monitoring in vivo reversible cysteine oxidation in proteins using ICAT and mass spectrometry. Nat. Protoc. 9:1131-1145.

Boronat, S., Domènech, A., Paulo, E., Calvo, I.A., García-Santamarina, S., García, P., Encinar del Dedo, J., Barcons, A., Serrano, E., Carmona, M. and Hidalgo, E. (2014) Thiol-based H2O2 signalling in microbial systems.  Redox Biol. 2:395-399.



Calvo, I.A., Boronat, S., Domènech, A., García-Santamarina, S., Ayté, J. and Hidalgo, E.  (2013)  Dissection of a redox relay: H2O2-dependent activation of the transcription factor Pap1 through the peroxidatic Tpx1-thioredoxin cycle.  Cell Reports 5:1413-1424.

García-Santamarina, S., Boronat, S., Ayté, J. and Hidalgo, E. (2013) Methionine sulfoxide reductases revisited: Free methionine as a primary target of H2O2 stress in auxotrophic fission yeast. Mol. Microbiol. 90:1113-1124.

Ivanova, T., Alves-Rodrigues, I., Gómez-Escoda, B., Dutta, C., DeCaprio, J.A., Rhind, N., Hidalgo, E. and Ayté, J. (2013) The DNA damage and the DNA replication checkpoints converge at the MBF transcription factor. Mol. Biol. Cell 24:3350-3357.

Molero, C., Petrényi, K., González, A., Carmona, M., Gelis, S., Abrie, A., Strauss, E., Ramos, J., Dombradi, V., Hidalgo, E. and Ariño, J. (2013) The Schizosaccharomyces pombe fusion gene hal3 encodes three distinct activities.  Mol. Microbiol.90:367-382.

Fernández-Vázquez, J., Vargas-Pérez, I., Sansó, M., Buhne, K., Carmona, M., Paulo, E., Hermand, D., Rodríguez-Gabriel, M., Ayté, J., Leidel, S. and Hidalgo, E. (2013) Modification of tRNA(Lys) UUU by Elongator is essential for efficient translation of stress mRNAs. PLoS Genet. 9(7):e1003647.

Calvo, I.A., Ayté, J. and Hidalgo, E. (2013) Reversible thiol oxidation in the H2O2-dependent activation of the transcription factor Pap1. J. Cell Sci. 126:2279-2284.

García-Santamarina, S., Boronat, S., Calvo, I.A., Rodríguez-Gabriel, M., Ayté, J., Molina, H. and Hidalgo, E. (2013) Is oxidized thioredoxin a major trigger for cysteine oxidation? Clues from a redox proteomics approach.  Antioxid. Redox Signal. 18:1549-1556.



Gabrielli, N., Ayté, J. and Hidalgo, E. (2012) Cells Lacking Pfh1, a Fission Yeast Homolog of Mammalian Frataxin, Display Constitutive Activation of the Iron Starvation Response. J. Biol. Chem. 287:43042-43051.

Guerra-Moreno, A., Alves-Rodrigues, I., Hidalgo, E. and Ayté, J. (2012) Chemical genetic induction of meiosis inSchizosaccharomyces pombe. Cell Cycle 11:1620-1624.

Calvo, I.A., García, P., Ayté, J. and Hidalgo, E. (2012) The transcription factors Pap1 and Prr1 collaborate to activate antioxidant, but not drug tolerance, genes in response to H2O2. Nucleic Acids Res. 40:4816-4824.



Sansó, M., Vargas-Pérez, I., García, P., Ayté, J. and Hidalgo, E. (2011) Nuclear roles and regulation of chromatin structure by the stress-dependent MAP kinase Sty1 of Schizosaccharomyces pombe. Mol. Microbiol. 82:542-554.

García-Santamarina, S., Boronat, S., Espadas, G., Ayté, J., Molina, H.  and Hidalgo, E. (2011) The oxidized thiol proteome in fission yeast - optimization of an ICAT-based method to identify H2O2-oxidized proteins.  J. Proteomics 74:2476-2486.

Sansó, M., Vargas-Pérez, I., Quintales, L., Antequera, F., Ayté, J. and Hidalgo, E. (2011) Gcn5 facilitates Pol II progression, rather than recruitment to nucleosome-depleted stress promoters, in Schizosaccharomyces pombe. Nucleic Acids Res. 39:6369-6379.

Ivanova, T., Gómez-Escoda, B., Hidalgo, E. and Ayté, J. (2011) G1/S transcription and the DNA-synthesis checkpoint: common regulatory mechanisms. Cell Cycle 10:912-915.

Gómez-Escoda, B., Ivanova, T., Calvo, I.A., Alves-Rodrigues, I., Hidalgo, E. and Ayté, J. (2011) Yox1 links MBF-dependent transcription to completion of DNA synthesis. EMBO Reports 12:84-89.


2010 and before (selected)

Zuin, A., Carmona, M., Morales-Ivorra, I., Gabrielli, N., Vivancos, A.P., Ayté, J. and Hidalgo, E. (2010) Life-span extension by calorie restriction relies on the Sty1 MAP kinase stress pathway. EMBO J. 29:981-991.

Calvo, I.A., Gabrielli, N., Iglesias-Baena, I., García-Santamarina, S., Hoe, K.L., Kim, D.U., Sansó, M., Zuin, A., Pérez, P., Ayté, J. and Hidalgo, E. (2009) Genome-wide screen of genes required for caffeine tolerance in fission yeast.  PLoS ONE4(8):e6619.

Moldón, A. and Ayté, J. (2009) At the (3') end, you'll turn to meiosis. Nat. Struct. Mol. Biol. 16:350-351.

Moldón, A., Malapeira, J., Gabrielli, N., Gogol, M., Gómez-Escoda, B., Ivanova, T., Seidel, C. and Ayté, J. (2008) Promoter-driven splicing regulation in fission yeast. Nature 455:997-1000.

Jara, M., Vivancos, A.P., Calvo, I.A., Moldón, A., Sansó, M. and Hidalgo, E. (2007) The peroxiredoxin Tpx1 is essential as a H2O2-scavenger during aerobic growth in fission yeast. Mol. Biol. Cell 18:2288-2295.

Vivancos, A.P., Jara, M., Zuin, A., Sansó, M. and Hidalgo, E. (2006) Oxidative stress in Schizosaccharomyces pombe: different H2O2 levels, different response pathways. Mol. Genet. Genomics 276:495-502.

Vivancos, A. P., Castillo, E.A., Biteau, B., Nicot, C., Ayté, J., Toledano, M.B. and Hidalgo, E. (2005) A cysteine-sulfinic acid in peroxiredoxin regulates H2O2-sensing by the antioxidant Pap1 pathway. Proc. Nat. Acad. Sci. USA 101:8875-8880.

Malapeira, J., Moldón, A., Hidalgo, E., Smith, G.R., Nurse, P. and Ayté, J. (2005) A meiosis-specific cyclin regulated bBy splicing Is required for proper progression through meiosis. Mol. Cell Biol. 25:6330-6337.

Vivancos, A., Castillo, E.A., Jones, N., Ayte, J. and Hidalgo, E. (2004) Activation of the redox-sensor Pap1 by hydrogen peroxide requires modulation of the intracellular oxidant concentration. Mol. Microbiol. 52: 1427-1435.

Castillo, E.A., Vivancos, A.P., Ayté, J., Jones, N. and Hidalgo, E. (2003) Schizosaccharomyces pombe cells lacking the Ran-binding protein Hba1 show a multidrug resistance phenotype due to constitutive nuclear accumulation of Pap1. J. Biol. Chem. 278:40565-40572

Castillo, E.A., Ayté, J., Chiva, C., Moldón, A., Carrascal, M., Abián, J., Jones, N. and Hidalgo, E. (2002) Diethylmaleate activates the transcription factor Pap1 by covalent modification of critical cysteine residues. Mol. Microbiol. 45:243-254.

Ayté, J. Schweitzer, C., Zarzov, P., Nurse, P. and DeCaprio, J.A. (2001) Feedback regulation of the MBF transcription factor by cyclin Cig2. Nature Cell Biol. 3:1043-1050.

Oxidative Stress and Cell Cycle Research Group

Molecular Medicine Program

Department of Experimental and Health Sciences

Edifici PRBB (campus del Mar)
Doctor Aiguader, 88
08003 Barcelona

 +34 933160847

[email protected]