Atrás Hidalgo, Elena


Departamento de Medicina y Ciencias de la Vida
Extrés Oxidativo y Ciclo Celular
  • 1981-1986. Graduate in Pharmacy in the School of Pharmacy at the University of Barcelona with a grade-point average of Excellent (A).
  • 1986-1987. Experimental work on the respiratory regulation of the enzyme L-1,2-propanediol oxidoreductase in Escherichia coli, directed by Dr. J. Aguilar at the Unit of Biochemistry, School of Pharmacy, University of Barcelona (Spain).
  • 1987-1991. Doctoral Thesis on "Characterization and regulation of the expression of the gene ald of Escherichia coli", directed by Dr. J. Aguilar at the Unit of Biochemistry, School of Pharmacy, University of Barcelona (Spain). Ph.D. in Biochemistry and Molecular Biology.
  • 1990-1992. Teaching Assistant Professor at the Unit of Biochemistry, School of Pharmacy, University of Barcelona.
  • 1992-1997. Postdoctoral fellow at the laboratory of Dr. Bruce Demple, Department of Molecular and Cellular Toxicology, Harvard School of Public Health, Harvard University, Boston.
  • 1998-1999. Postdoctoral fellow at the laboratory of Dr. Nic Jones, Gene Regulation Laboratory, Imperial Cancer Reseach Fund, London.
  • 1999-present     Professor at Universitat Pompeu Fabra, Barcelona, Spain.


Sánchez-Mir, L., Salat-Canela, C., Paulo, E., Carmona, M., Ayté, J., Oliva, B. and Hidalgo E. (2018). Phospho-mimicking Atf1 mutants bypass the transcription activating function of the MAP kinase Sty1 of fission yeast. Curr. Genet. 64:97-102.

Salat-Canela, C., Paulo, E., Sánchez-Mir, L., Carmona, M., Ayté, J., Oliva, B. and Hidalgo E. (2017). Deciphering the role of the signal- and Sty1 kinase-dependent phosphorylation of the stress-responsive transcription factor Atf1 on gene activation. J. Biol. Chem. 292:13635-13644.

Boronat, S., Domènech, A., Carmona, M., García-Santamarina, S., Bañó, M.C., Ayté, J. and Hidalgo, E. (2017). Lack of a peroxiredoxin suppresses the lethality of cells devoid of electron donors by channelling electrons to oxidized ribonucleotide reductase.  PLoS Genet. 13(6):e1006858.

Boronat, S., Domènech, A. and Hidalgo, E. (2017) Proteomic characterization of reversible thiol oxidations in proteomes and proteins. Antioxid. Redox Signal. 26:329-344.

Shah, M., Su, D., Scheliga, J.S., Pluskal, T., Boronat, S., Motamedchaboki, K., Rosa Campos, A., Qi, F., Hidalgo, E., Yanagida, M. and Wolf, D.A. (2016) A Transcript-Specific eIF3 Complex Mediates Global Translational Control of Energy Metabolism. Cell Reports 16:1891-902.

Gaspa, L., González-Medina, A., Hidalgo, E. and Ayté, J. (2016) A functional genome-wide genetic screening identifies new pathways controlling the G1/S transcriptional wave. Cell Cycle 15:720-72.

Alves-Rodrigues, I., Ferreira, P.G., Moldón, A., Vivancos, A.P., Hidalgo, E., Guigó, R and Ayté, J. (2016) Spatiotemporal control of forkheads binding to DNA regulates the meiotic gene expression program. Cell Reports 14:885-895.

García, P., Encinar del Dedo, J., Ayté, J. and Hidalgo, E. (2016) Genome-wide Screening of Regulators of Catalase Expression: Role of a Transcription Complex and Histone and tRNA Modification Complexes on Adaptation to Stress. J. Biol. Chem. 291:790-799.

Vázquez, B., Soto, T., Del Dedo, J.E., Franco, A., Vicente, J., Hidalgo, E., Gacto, M., Cansado, J. and Madrid, M. (2015) Distinct biological activity of threonine monophosphorylated MAPK isoforms during the stress response in fission yeast. Cell Signal. 27:2534-2542.

Materne, P., Anandhakumar, J., Migeot, V., Soriano, I., Yague-Sanz, C., Hidalgo, E., Mignion, C., Quintales, L., Antequera, F. and Hermand, D. (2015) Promoter nucleosome dynamics regulated by signaling through the CTD code.  eLife 4:e09008.

Encinar del Dedo, J., Gabrielli, N., Carmona, M., Ayté, J., and Hidalgo, E. (2015) A cascade of iron-containing proteins governs the genetic iron starvation response to promote iron uptake and inhibit iron storage in fission yeast.  PLoS Genet. 11(3):e1005106.

Boronat, S., García-Santamarina, S. and Hidalgo, E.  (2015) Gel-free proteomic methodologies to study reversible cysteine oxidation and irreversible protein carbonyl formation.  Free Radic. Res. 49:494-510.

García, P., Paulo, E., Gao, J., Wahls, W., Ayté, J., Lowy, E. and Hidalgo, E. (2014) Binding of the transcription factor Atf1 to promoters serves as a barrier to phase nucleosome arrays and avoid cryptic transcription. Nucleic Acids Res. 42:10351-10359.

García-Santamarina, S., Boronat, S. and Hidalgo, E. (2014) Reversible cysteine oxidation in hydrogen peroxide sensing and signal transduction. Biochemistry 53:2560:2580.

Paulo, E., García-Santamarina, S., Calvo, I.A., Carmona, M., Boronat, S., Domènech, A., Ayté, J. and Hidalgo, E. (2014) A genetic approach to study H2O2 scavenging in fission yeast - distinct roles of peroxiredoxin and catalase.  Mol. Microbiol. 92:246-257.

García-Santamarina, S., Boronat, S., Domènech, A., Ayté, J., Molina, H. and Hidalgo, E. (2014) Monitoring in vivo reversible cysteine oxidation in proteins using ICAT and mass spectrometry. Nat. Protoc. 9:1131-1145.

Boronat, S., Domènech, A., Paulo, E., Calvo, I.A., García-Santamarina, S., García, P., Encinar del Dedo, J., Barcons, A., Serrano, E., Carmona, M. and Hidalgo, E.  (2014) Thiol-based H2O2 signalling in microbial systems.  Redox Biol. 2:395-399.

Calvo, I.A., Boronat, S., Domènech, A., García-Santamarina, S., Ayté, J. and Hidalgo, E.  (2013)  Dissection of a redox relay: H2O2-dependent activation of the transcription factor Pap1 through the peroxidatic Tpx1-thioredoxin cycle. Cell Reports 5:1413-1424.

García-Santamarina, S., Boronat, S., Ayté, J. and Hidalgo, E. (2013) Methionine sulfoxide reductases revisited: Free methionine as a primary target of H2O2 stress in auxotrophic fission yeast. Mol. Microbiol. 90:1113-1124.

Ivanova, T., Alves-Rodrigues, I., Gómez-Escoda, B., Dutta, C., DeCaprio, J.A., Rhind, N., Hidalgo, E. and Ayté, J. (2013) The DNA damage and the DNA replication checkpoints converge at the MBF transcription factor. Mol. Biol. Cell 24:3350-3357.

Molero, C., Petrényi, K., González, A., Carmona, M., Gelis, S., Abrie, A., Strauss, E., Ramos, J., Dombradi, V., Hidalgo, E. and Ariño, J. (2013) The Schizosaccharomyces pombe fusion gene hal3 encodes three distinct activities.  Mol. Microbiol. 90:367-382.

Fernández-Vázquez, J., Vargas-Pérez, I., Sansó, M., Buhne, K., Carmona, M., Paulo, E., Hermand, D., Rodríguez-Gabriel, M., Ayté, J., Leidel, S. and Hidalgo, E. (2013) Modification of tRNA Lys UUU by Elongator is essential for efficient translation of stress mRNAs. PLoS Genet. 9(7):e1003647

Calvo, I.A., Ayté, J. and Hidalgo, E. (2013) Reversible thiol oxidation in the H2O2-dependent activation of the transcription factor Pap1. J. Cell Sci. 126:2279-2284.

García-Santamarina, S., Boronat, S., Calvo, I.A., Rodríguez-Gabriel, M., Ayté, J., Molina, H. and Hidalgo, E. (2013) Is oxidized thioredoxin a major trigger for cysteine oxidation? Clues from a redox proteomics approach.  Antioxid. Redox Signal. 18:1549-1556.

Gabrielli, N., Ayté, J. and Hidalgo, E. (2012) Cellsl acking Pfh1, a fission yeast homolog of mammalian frataxin, display constitutive activation of the iron starvation response. J. Biol. Chem. 287:43042-43051.

Guerra-Moreno, A., Alves-Rodrigues, I., Hidalgo, E. and Ayté, J. (2012) Chemical genetic induction of meiosis inSchizosaccharomyces pombe. Cell Cycle 11:1620-1624.

Calvo, I.A., García, P., Ayté, J. and Hidalgo, E. (2012) The transcription factors Pap1 and Prr1 collaborate to activate antioxidant, but not drug tolerance, genes in response to H2O2. Nucleic Acids Res. 40:4816-4824.

Sansó, M., Vargas-Pérez, I., García, P., Ayté, J. and Hidalgo, E. (2011) Nuclear roles and regulation of chromatin structure by the stress-dependent MAP kinase Sty1 of Schizosaccharomyces pombe. Mol. Microbiol. 82:542-554.

García-Santamarina, S., Boronat, S., Espadas, G., Ayté, J., Molina, H.  and Hidalgo, E. (2011) The oxidized thiol proteome in fission yeast - optimization of an ICAT-based method to identify H 22-oxidized proteins.  J. Proteomics 74:2476-2486.

Sansó, M., Vargas-Pérez, I., Quintales, L., Antequera, F., Ayté, J. and Hidalgo, E. (2011) Gcn5 facilitates Pol II progression, rather than recruitment to nucleosome-depleted stress promoters, in Schizosaccharomyces pombe. Nucleic Acids Res. 39:6369-6379.

Ivanova, T., Gómez-Escoda, B., Hidalgo, E. and Ayte, J. (2011) G1/S transcription and the DNA-synthesis checkpoint: common regulatory mechanisms. Cell Cycle 10:912-915.

Gómez-Escoda, B., Ivanova, T., Calvo, I.A., Alves-Rodrigues, I., Hidalgo, E. and Ayte, J. (2011) Yox1 links MBF-dependent transcription to completion of DNA synthesis. EMBO Reports 12:84-89.

Zuin, A., Castellano-Esteve, D., Ayté, J and Hidalgo, E. (2010) Living on the edge: stress and activation of stress responses promote lifespan extension. Aging 2(4):231-237.

Zuin, A., Carmona, M., Morales-Ivorra, I., Gabrielli, N., Vivancos, A.P., Ayté, J. and Hidalgo, E. (2010) Life-span extension by calorie restriction relies on the Sty1 MAP kinase stress pathway. EMBO J. 29:981-991. 

Calvo, I.A., Gabrielli, N., Iglesias-Baena, I., García-Santamarina, S., Hoe, K.L., Kim, D.U., Sansó, M., Zuin, A., Pérez, P., Ayté, J. and Hidalgo, E. (2009) Genome-wide screen of genes required for caffeine tolerance in fission yeast. PLoS ONE 4(8):e6619.

Zuin, A., Calvo, I.A., Gabrielli, N., García-Santamaría, S., Hoe, K.-L., Kim, D.U., Park, H.-O., Hayles, J., Ayté, J. and Hidalgo, E. (2008) Mitochondrial dysfunction increases oxidative stress and decreases chronological life span in fission yeast. PLoS ONE 3:e2842.

Sansó, M., Gogol, M, Ayté, J., Seidel, C. and Hidalgo, E. (2008) The transcription factors Pcr1 and Atf1 have distinct roles in stress- and Sty1-dependent gene regulation. Eukaryot. Cell 7:826-835.

López-Avilés, S., Lambea, E., Moldón, A., Grande, M., Fajardo, A., Rodriguez-Gabriel, M.A., Hidalgo, E. and Aligue, R. (2008) Activation of Srk1 by the MAP kinase Sty1 precedes its dissociation from the kinase and signals its degradation. Mol. Biol. Cell 19:1670-1679.

Jara, M., Vivancos, A.P. and Hidalgo, E. (2008) C-terminal truncation of the peroxiredoxin Tpx1 decreases its sensitivity for hydrogen peroxide without compromising its role in signal transduction. Genes to Cells 13:171-9.

Jara, M., Vivancos, A.P., Calvo, M.I., Moldón, A., Sansó, M and Hidalgo, E.  (2007)  The peroxirredoxin Tpx1 is essential as a H2O2-scavenger during aerobic growth in fission yeast.  Mol. Biol. Cell 18:2288-2295.

Vivancos, A.P., Jara, M., Zuin,  A., Sansó, M. and Hidalgo, E. (2006) Oxidative stress in Schizosaccharomyces pombe: different H2O2 levels, different response pathways.  Mol. Genet. Genomics 276:495-502.

Zuin,  A., Vivancos, A.P., Sansó, M., Takatsume, Y., Ayté, J., Inoue, Y. and Hidalgo, E. (2005) The glycolytic metabolite methylglyoxal activates Pap1 and Sty1 stress responses in Schizosaccharomyces pombe. J. Biol. Chem. 280:36708-36713.

Vivancos, A. P., Castillo, E.A., Biteau, B., Nicot, C., Ayté, J., Toledano, M.B. and Hidalgo, E. (2005) A cysteine-sulfinic acid in peroxiredoxin regulates H2O2-sensing by the antioxidant Pap1 pathway.  Proc. Nat. Acad. Sci. USA 101:8875-8880.

Malapeira, J., Moldón, A., Hidalgo, E., Smith, G.R., Nurse, P. and Ayté, J. (2005) A Meiosis-Specific Cyclin Regulated By Splicing Is Required For Proper Progression Through Meiosis.  Mol. Cell Biol. 25:6330-6337.

Madrid, M., Soto, T., Franco, A., Paredes, V., Vicente, J. Hidalgo, E., Gacto, M. and Cansado, J. (2004) A cooperative role for Atf1 and Pap1 in the detoxification of oxidative stress induced by glucose deprivation in Schizosaccharomyces pombe.  J. Biol. Chem. 279:41594-602.

Vivancos, A., Castillo, E.A., Jones, N., Ayte, J. and Hidalgo, E. (2004) Activation of the redox-sensor Pap1 by hydrogen peroxide requires modulation of the intracellular oxidant concentration.  Mol. Microbiol. 52: 1427-1435.

Castillo, E.A., Vivancos, A.P. and Hidalgo, E. (2003) Pap1, a sensor of oxidative stress in Schizosaccharomyces pombe.  In Recent Developments in Cell Research (Pandalai, S.G., ed), Research Signpost, Trivandrum, p.217-230.

Castillo, E.A., Vivancos, A., Ayté, J., Jones, N. and Hidalgo, E. (2003) Schizosaccharomyces pombe cells lacking the Ran-binding protein Hba1 show a multidrug resistance phenotype due to constitutive nuclear accumulation of Pap1.  J. Biol. Chem. 278:40565-40572.

Castillo, E.A., Ayté, J., Chiva, C., Moldón, A., Carrascal, M., Abián, J., Jones, N. and Hidalgo, E. (2002) Diethylmaleate activates the transcription factor Pap1 by covalent modification of critical cysteine residues. Mol. Microbiol. 45:243-254.

Sánchez-Piris, M., Posas, F., Alemany, V., Winge, I., Hidalgo, E., Bachs, O. and Aligué, R. (2002) The serine/threonine kinase Cmk2 is required for oxidative stress response in fission yeast. J. Biol. Chem. 277:17722-17727.

Demple, B., Hidalgo, E. and Ding, H. (1999) Transcriptional regulation via redox-sensitive iron-sulphur centres in an oxidative stress response. Biochem. Soc. Symp. 64:119-128. 

Hidalgo, E., Leautaud, V. and Demple, B. (1998) The redox-regulated SoxR protein acts from a single DNA site as a repressor and an allosteric activator. EMBO J. 17:2629-2636.

Hidalgo, E., Ding, H. and Demple, B. (1997) Redox signal transduction via iron-sulfur clusters in transcription factors. Trends Biochem. Sci. 22:207-210.

Bradley, T.M., Hidalgo, E., Leautaud, V., Ding, H. and Demple, B. (1997) Cysteine-to-alanine replacements in the E. coli SoxR protein and the role of the [2Fe-2S] centers in transcriptional activation. Nucleic Acids Res. 25:1469-1475.

Limón, A., Hidalgo, E. and Aguilar, J. (1997) The ald gene of Escherichia coli is under the control of at least three transcriptional regulators. Microbiology 143:2085-2095.

Hidalgo, E. and Demple, B. (1997) Spacing of promoter elements regulates the basal expression of the soxS gene and converts SoxR from a transcriptional activator into a repressor. EMBO J. 16:1056-1065.

Hidalgo, E., Ding, H. and Demple, B. (1997) Redox signal transduction: mutations shifting [2Fe-2S] centers of the SoxR sensor-regulator to the oxidized form. Cell 88:121-129.

Ding, H., Hidalgo, E. and Demple, B. (1996) The redox state of the [2Fe-2S] clusters in SoxR protein regulates its activity as a transcription factor. J. Biol. Chem. 52:33173-33175.

Hidalgo, E., Limon, A. and Aguilar, J. (1996) A second Escherichia coli gene with similarity to gapA. Microbiologia 12:99-106.

Hidalgo, E. and Demple, B. (1996) Activation of SoxR-dependent transcription in vitro by noncatalytic or NifS-mediated assembly of [2Fe-2S] clusters into apo-SoxR. J. Biol. Chem. 271:7269-7272.

Hidalgo, E. and Demple, B. (1997) Adaptive responses to oxidative stress: the soxRS and oxyR regulon, in Regulation of Gene Expression in Escherichia coli (Lin, E.C.C. and Lynch, S., eds) R.G. Landes Company, Austin, pp.435-452.

Hidalgo, E., Bollinger, J. M.Jr., Bradley, T. M., Walsh, C.T. and Demple, B. (1995) Binuclear [2Fe-2S] clusters in the Escherichia coli  SoxR protein and role of the metal centers in transcription. J. Biol. Chem. 270:20908-20914.

Hidalgo, E., Nunoshiba, T. and Demple, B. (1994) Molecular genetics of the soxRS oxidative stress regulon of Escherichia coli.. Meth. Mol. Genet. 3:325-339.

Hidalgo, E. and Demple, B. (1994) An iron-sulfur center essential for transcriptional activation by the redox-sensing SoxR protein. EMBO J. 10:138-146.

Nunoshiba, T., Hidalgo, E., Li, Z. and Demple, B. (1993) Negative autoregulation by the Escherichia coli SoxS protein: a dampening mechanism for the soxRS redox stress response. J. Bacteriol. 175:7492-7494.

Moralejo, P, Egan, S.M., Hidalgo, E. and Aguilar, J. (1993) Sequencing and characterization  of a gene cluster encoding the enzymes for L-rhamnose metabolism in Escherichia coli.. J. Bacteriol. 175:5585-5594.

Nunoshiba, T., Hidalgo, E., Amábile-Cuevas, C.F. and Demple, B. (1992) Two-stage control of an oxidative stress regulon: the Escherichia coli SoxR protein triggers redox-inducible expression of the soxS regulatory gene. J. Bacteriol. 174:6054-6060.

Cabiscol, E., Badía, J., Baldomá, L., Hidalgo, E., Aguilar, J. and Ros, J. (1992) Inactivation of propanediol oxidoreductase of Escherichia coli by metal-catalyzed oxidation. Biochim. et Biophys. Acta 1118:155-160.

Hidalgo, E., Chen, Y.-M., Lin, E.C.C. and Aguilar, J. (1991) Molecular cloning and DNA sequencing of the Escherichia coli K-12 ald gene encoding aldehyde dehydrogenase. J. Bacteriol. 173:6118-6123.

Cabiscol, E., Hidalgo, E., Badía, J., Baldomá, L., L., Ros, J. and Aguilar, J. (1990) Oxygen regulation of L-1,2-propanediol oxidoreductase activity in Escherichia coli. J. Bacteriol. 172:5514-5515.



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