Atrás Ayte, Jose


Departamento de Medicina y Ciencias de la Vida
Extrés Oxidativo y Ciclo Celular
Personal profile in our Scientific Output Portal (PPC)


  • 1980-1985: Graduate in Pharmacy at the University of Barcelona with a grade-point average of Excellent (3).
  • 1986-1990: Doctoral Thesis on "Mitochondrial and cytosolic HMG-CoA synthases are encoded by two different genes", directed by Dr. Fausto García Hegardt at the Department of Biochemistry, School of Pharmacy, University of Barcelona (Spain). Ph.D. in Biochemistry and Molecular Biology.
  • 1992-1997: Postdoctoral Fellow.  Supervisor: James A. DeCaprio, Dana-Farber Cancer Institute and Harvard Medical School, Boston, USA.  The research topic was “Cell cycle control in Schizosaccharomyces pombe”.
  • 1998-1999: Postdoctoral fellow.  Supervisor: Paul Nurse, Imperial Cancer Research Fund, London, UK.  The research topic was “Cell cycle control in Schizosaccharomyces pombe”.
  • 1999-present: Professor at Universitat Pompeu Fabra, Barcelona, Spain.



Domènech, A., Ayté, J., Antunes, F. and Hidalgo, E. (2018). Using in vivo oxidation status of one- and two-component redox relays to determine H2O2 levels linked to signaling and toxicity. BMC Biol. 16:61.

Sánchez-Mir, L., Salat-Canela, C., Paulo, E., Carmona, M., Ayté, J., Oliva, B. and Hidalgo E. (2018). Phospho-mimicking Atf1 mutants bypass the transcription activating function of the MAP kinase Sty1 of fission yeast. Curr. Genet. 64:97-102.

Salat-Canela, C., Paulo, E., Sánchez-Mir, L., Carmona, M., Ayté, J., Oliva, B. and Hidalgo E. (2017). Deciphering the role of the signal- and Sty1 kinase-dependent phosphorylation of the stress-responsive transcription factor Atf1 on gene activation. J. Biol. Chem. 292:13635-13644.

Boronat, S., Domènech, A., Carmona, M., García-Santamarina, S., Bañó, M.C., Ayté, J. and Hidalgo, E. (2017). Lack of a peroxiredoxin suppresses the lethality of cells devoid of electron donors by channelling electrons to oxidized ribonucleotide reductase.  PLoS Genet. 13(6):e1006858.

Alves-Rodrigues, I., Ferreira, P.G., Moldón, A., Vivancos, A.P., Hidalgo, E., Guigó, R and Ayté, J. (2016) Spatiotemporal control of forkheads binding to DNA regulates the meiotic gene expression program. Cell Reports 14:885-895. 

Gaspa, L., González-Medina, A., Hidalgo, E. and Ayté, J. (2016) A functional genome-wide genetic screening identifies new pathways controlling the G1/S transcriptional wave. Cell Cycle 15:720-72.

Eckert, D., Andrée, N., Razanau, A., Zock-Emmenthal, S., Lützelberger, M., Plath, S., Schmidt, H., Guerra-Moreno, A., Cozzuto, L., Ayté, J. and Käufer, N.F. (2016) Prp4 kinase grants the license to splice: control of weak splice sites during spliceosome activation. PLoS Genet. 12(1):e1005768.

García, P., Encinar del Dedo, J., Ayté, J. and Hidalgo, E. (2016) Genome-wide Screening of Regulators of Catalase Expression: Role of a Transcription Complex and Histone and tRNA Modification Complexes on Adaptation to Stress. J. Biol. Chem. 291:790-799.

Encinar del Dedo, J., Gabrielli, N., Carmona, M., Ayté, J., and Hidalgo, E. (2015) A cascade of iron-containing proteins governs the genetic iron starvation response to promote iron uptake and inhibit iron storage in fission yeast.  PLoS Genet. 11(3):e1005106.

García, P., Paulo, E., Gao, J., Wahls, W., Ayté, J., Lowy, E. and Hidalgo, E. (2014) Binding of the transcription factor Atf1 to promoters serves as a barrier to phase nucleosome arrays and avoid cryptic transcription. Nucleic Acids Res. 42:10351-10359.

Paulo, E., García-Santamarina, S., Calvo, I.A., Carmona, M., Boronat, S., Domènech, A., Ayté, J. and Hidalgo, E. (2014) A genetic approach to study H2O2 scavenging in fission yeast - distinct roles of peroxiredoxin and catalase.  Mol. Microbiol. 92:246-257.

García-Santamarina, S., Boronat, S., Domènech, A., Ayté, J., Molina, H. and Hidalgo, E. (2014) Monitoring in vivo reversible cysteine oxidation in proteins using ICAT and mass spectrometry. Nat. Protoc. 9:1131-1145.

Calvo, I.A., Boronat, S., Domènech, A., García-Santamarina, S., Ayté, J. and Hidalgo, E.  (2013)  Dissection of a redox relay: H2O2-dependent activation of the transcription factor Pap1 through the peroxidatic Tpx1-thioredoxin cycle. Cell Reports 5:1413-1424.

García-Santamarina, S., Boronat, S., Ayté, J. and Hidalgo, E. (2013) Methionine sulfoxide reductases revisited: Free methionine as a primary target of H2O2 stress in auxotrophic fission yeast. Mol. Microbiol. 90:1113-1124.

Ivanova, T., Alves-Rodrigues, I., Gómez-Escoda, B., Dutta, C., DeCaprio, J.A., Rhind, N., Hidalgo, E. and Ayté, J. (2013) The DNA damage and the DNA replication checkpoints converge at the MBF transcription factor. Mol. Biol. Cell 24:3350-3357.

Fernández-Vázquez, J., Vargas-Pérez, I., Sansó, M., Buhne, K., Carmona, M., Paulo, E., Hermand, D., Rodríguez-Gabriel, M., Ayté, J., Leidel, S. and Hidalgo, E. (2013) Modification of tRNA Lys UUU by Elongator is essential for efficient translation of stress mRNAs. PLoS Genet. 9(7):e1003647.

Calvo, I.A., Ayté, J. and Hidalgo, E. (2013) Reversible thiol oxidation in the H2O2-dependent activation of the transcription factor Pap1. J. Cell Sci. 126:2279-2284.

García-Santamarina, S., Boronat, S., Calvo, I.A., Rodríguez-Gabriel, M., Ayté, J., Molina, H. and Hidalgo, E. (2013)  Is oxidized thioredoxin a major trigger for cysteine oxidation? Clues from a redox proteomics approach.  Antioxid. Redox Signal. 18:1549-1556.

Gabrielli, N., Ayté, J. and Hidalgo, E. (2012) Cellsl acking Pfh1, a fission yeast homolog of mammalian frataxin, display constitutive activation of the iron starvation response. J. Biol. Chem. 287:43042-43051.

Guerra-Moreno, A., Alves-Rodrigues, I., Hidalgo, E. and Ayté, J. (2012) Chemical genetic induction of meiosis inSchizosaccharomyces pombe. Cell Cycle 11:1620-1624.

Calvo, I.A., García, P., Ayté, J. and Hidalgo, E. (2012) The transcription factors Pap1 and Prr1 collaborate to activate antioxidant, but not drug tolerance, genes in response to H2O2. Nucleic Acids Res. 40:4816-4824.

Sansó, M., Vargas-Pérez, I., García, P., Ayté, J. and Hidalgo, E. (2011) Nuclear roles and regulation of chromatin structure by the stress-dependent MAP kinase Sty1 of Schizosaccharomyces pombe. Mol. Microbiol. 82:542-554.

García-Santamarina, S., Boronat, S., Espadas, G., Ayté, J., Molina, H.  and Hidalgo, E. (2011) The oxidized thiol proteome in fission yeast - optimization of an ICAT-based method to identify H 22-oxidized proteins.  J. Proteomics 74:2476-2486.

Sansó, M., Vargas-Pérez, I., Quintales, L., Antequera, F., Ayté, J. and Hidalgo, E. (2011) Gcn5 facilitates Pol II progression, rather than recruitment to nucleosome-depleted stress promoters, in Schizosaccharomyces pombe. Nucleic Acids Res. 39:6369-6379.

Ivanova, T., Gómez-Escoda, B., Hidalgo, E. and Ayte, J. (2011) G1/S transcription and the DNA-synthesis checkpoint: common regulatory mechanisms. Cell Cycle 10:912-915.

Gómez-Escoda, B., Ivanova, T., Calvo, I.A., Alves-Rodrigues, I., Hidalgo, E. and Ayte, J. (2011) Yox1 links MBF-dependent transcription to completion of DNA synthesis. EMBO Reports 12:84-89.

Zuin, A., Castellano-Esteve, D., Ayté, J and Hidalgo, E. (2010) Living on the edge: stress and activation of stress responses promote lifespan extension. Aging 2(4):231-237.

Zuin, A., Carmona, M., Morales-Ivorra, I., Gabrielli, N., Vivancos, A.P., Ayté, J. and Hidalgo, E. (2010) Life-span extension by calorie restriction relies on the Sty1 MAP kinase stress pathway. EMBO J. 29:981-991. 

Calvo, I.A., Gabrielli, N., Iglesias-Baena, I., García-Santamarina, S., Hoe, K.L., Kim, D.U., Sansó, M., Zuin, A., Pérez, P., Ayté, J. and Hidalgo, E. (2009) Genome-wide screen of genes required for caffeine tolerance in fission yeast. PLoS ONE 4(8):e6619.

Moldón, A. and Ayté, J. (2009) At the (3') end, you'll turn to meiosis. Nat. Struct. Mol. Biol. 16:350-351.

Moldón, A., Malapeira, J., Gabrielli, N., Gogol, M., Gómez-Escoda, B., Ivanova, T., Seidel, C. and Ayté, J. (2008) Promoter-driven splicing regulation in fission yeast. Nature 455:997-1000.

Zuin, A., Calvo, I.A., Gabrielli, N., García-Santamaría, S., Hoe, K.-L., Kim, D.U., Park, H.-O., Hayles, J., Ayté, J. and Hidalgo, E. (2008) Mitochondrial dysfunction increases oxidative stress and decreases chronological life span in fission yeast. PLoS ONE 3:e2842.

Sansó, M., Gogol, M., Ayté, J., Seidel, C. and Hidalgo, E. (2008) The transcription factors Pcr1 and Atf1 have distinct roles in stress- and Sty1-dependent gene regulation. Eukaryot. Cell 7:826-835.

Malapeira, J., Moldón, A., Hidalgo, E., Smith, G.R., Nurse, P. and Ayté, J. (2005) A meiosis-specific cyclin regulated by splicing is required for proper progression through meiosis.  Mol. Cell Biol. 25: 6330-6337.

Zuin, A., Vivancos, A.P., Sansó, M., Takatsume, Y., Ayté, J., Inoue, Y. and Hidalgo, E. (2005) The glycolytic metabolite methylglyoxal activates Pap1 and Sty1 stress responses in Schizosaccharomyces pombe.  J. Biol.. Chem  280:36708-36713.

Vivancos, A.P., Castillo, E.A., Biteau, B., Nicot, C., Ayté, J., Toledano, M.B. and Hidalgo, E. (2005)  A cysteine-sulfinic acid in peroxiredoxin regulates H2O2-sensing by the antioxidant Pap1 pathway. Proc. Natl. Acad. Sci. USA. 102:8875-8880.

Vivancos, A.P., Castillo, E.A., Jones, N., Ayté, J. and Hidalgo, E. (2004) Activation of the redox sensor Pap1 by hydrogen peroxide requires modulation of the intracellular oxidant concentration. Mol. Microbiol.  52: 1427-35.

Castillo, E.A., Vivancos, A.P., Jones, N., Ayté, J. and Hidalgo, E. (2003) Schizosaccharomyces pombe cells lacking the Ran-binding protein Hba1 show a multidrug resistance phenotype due to constitutive nuclear accumulation of Pap1. J. Biol. Chem. 278:40565-72.

*Castillo, E.A, *Ayté, J., Moldón, A., Jones, N. and Hidalgo, E. (2002) Hydrogen peroxide and diethylmaleate activate the transcription factor Pap1 by independent redox mechanisms. Mol. Microbiol. 45: 243-54.  *Co-authors

Borgne, A., Murakami, H., Ayté, J. and Nurse, P. (2002) The G1/S cyclin Cig2p during meiosis in fission yeast.  Mol. Biol. Cell 13:2080-2090.

Ayté, J., Schweitzer, C., Zarzov, P., Nurse, P. and DeCaprio, J.A. (2001) Feedback regulation of  the MBF transcription factor by ciclin cig2.  Nature Cell Biol. 3:1043-1050.

Ayté, J., Leis, J. and DeCaprio, J.A. (1997)  The fission yeast protein p73res2 is an essential component of the mitotic MBF complex and a regulator of meiosis. Mol. Biol. Cell 17: 6246-54.

Ayté, J., Leis, J., Herrera, A., Tang, E., Yang, H. and DeCaprio, J.A. (1995)  The Schizosaccharomyces pombe MBF complex requires heterodimerization for entry into S phase. Mol. Cell. Biol. 15: 2589-2599.

Royo, T., Pedragosa, M.J., Ayté, J., Gil-Gómez, G., Vilaró, S. and Hegardt, F.G. (1995) Immunolocalization of mitochondrial 3-hydroxy-3-methylglutaryl CoA synthase in rat liver. J. Cell Physiol. 162: 103-109.

Asins, G., Rosa, J.L., Serra, D., Gil-Gómez, G., Ayté, J., Bartons, R., Tauler, A. and Hegardt, F.G. (1994) Gene expression of enzymes regulating ketogenesis and fatty acid metabolism in regenerating rat liver. Biochem. J. 299:65-69.

Royo, T., Pedragosa, M.J., Ayté, J., Gil-Gómez, G., Vilaró, S. and Hegardt, F.G. (1993) Testis and ovary express the gene for the ketogenic mitochondrial-3-hydroxy-3-methylglutaryl-CoA synthase. J. Lipid Res. 34: 867-874.

Ayté, J., Gil-Gómez, G. and Hegardt, F.G. (1993) Methylation pattern of the mitochodrial 3-hydroxy-3-methylglutaryl coenzyme A synthase promoter and its 5' flanking region during development. Biochem. J. 295: 807-812.

Gil-Gómez, G., Ayté, J. and Hegardt, F.G. (1993) The rat mitochondrial 3-hydroxy-3-methylglutaryl coenzyme A synthase gene contains elements that mediate its multihormonal regulation and tissue-specificity. Eur. J. Biochem. 213: 773-779.

Ayté, J., Gil-Gómez, G. and Hegardt, F.G. (1993) Structural characterization of the 3' non-coding region of the gene encoding rat mitochondrial 3-hydroxy-3- methylglutaryl Coenzyme A synthase. Gene 123: 267-270.

Casals, N., Roca, N., Guerrero, M., Gil-Gómez, G., Ayté, J. Ciudad, C.J. and Hegardt, F.G. (1992) Regulation of the expresión of the mitochondrial HMG-CoA synthase gene. Biochem. J. 283: 261-264.

Royo, T., Ayté, J., Albericio, F., Giralt, E., Haro, D. and Hegardt, F.G. (1991) Diurnal rhythm of rat liver cytosolic 3-hydroxy-3-methylglutaryl-CoA synthase. Biochem. J. 15: 61-64.

Gil-Gomez, G., Marrero, P.F., Haro, D., Ayté, J. and Hegardt, F.G. (1991) Characterization of the gene encoding the 10 kDa polypeptide of photosystem II from Arabidopsis thaliana. Plant Mol. Biol. 17: 517-522.

Ayté, J., Gil-Gomez, G. and Hegardt, F.G. (1990) Nucleotide sequence of a rat liver cDNA encoding the cytosolic 3-hydroxy-3-methylglutaryl coenzyme A synthase. 18:3642.

Ayté, J., Gil-Gomez, G., Haro, D., Marrero, P.F. and Hegardt, F.G. (1990) Rat mitochondrial and cytosolic 3-hydroxy-3-methylglutaryl-CoA synthases are encoded by two different genes. Proc. Natl. Acad. Sci. USA. 87:3874-3878.

Haro, D, Marrero, P.F., Ayte, J. aad Hegardt, F.G. (1990) Identification of a cholesterol-regulated 180-kDa microsomal protein in rat hepatocytes. Eur. J. Biochem. 188:123-129.