Protein-protein interactions

  • Setting up of a protein-protein interaction experiment (IP, AP-MS)
  • Characterization of protein-protein interactions (IP, AP-MS)

Post-translational modifications

  • Acetyl, methyl, Gly-Gly PTM identification and quantification of a purified protein (LC or IP)
  • Identification and quantitation of post-translational modifications in purified histones.
  • Quantitation of post-translational modifications in purified histones.
  • Phosphorylation PTM identification of a purified protein (LC or IP)

Label-free (phospho-)proteome quantitation

  • Label-free protein quantitation in complex samples with and without statistical analysis
  • Label-free phosphopeptide quantitation in complex samples with and without statistical analysis

Label-based (phospho-)proteome quantitation

  • Protein quantitation in complex samples by SILAC with and without statistical analysis
  • Protein quantitation in complex samples by iTRAQ with and without statistical analysis
  • Protein quantitation in complex samples by TMT with and without statistical analysis
  • Phosphopeptide quantitation in complex samples by SILAC with and without statistical analysis

Single-protein analysis

  • Confirmation of an overexpressed protein in complex samples
  • Identification of a protein in a gel band.
  • Molecular weight determination of intact protein

Targeted Proteomics

  • Peptide selection for targeted proteomics quantitation with SRM
  • Optimization of SRM methods for targeted protein quantitation
  • Targeted protein quantitation with SRM with and without statistical analysis
  • Peptide selection for targeted proteomics quantitation with PRM
  • Optimization of PRM methods for targeted protein quantitation
  • Targeted protein quantitation with PRM with and without statistical analysis
  • Peptide selection for targeted proteomics quantitation with DIA
  • Optimization of DIA methods for targeted protein quantitation
  • Targeted protein quantitation with DIA with and without statistical analysis