Using quantitative data in single cells and mutational analyses, we study the role of transcriptional regulation in cell survival upon stress. We are interested in (1) identifying new transcriptional targets of cellular adaptation to stress, uncovering the mechanisms by which MAP kinases regulate mRNA biogenesis; (2) studying the relevance of histone modifications in stress adaptation; and (3) characterizing gene expression variability and cellular fitness in single cells upon stress, performing systematic genetic perturbations to identify transcriptional drivers of heterogeneity. Our main aim is to determine the contribution of multiple factors to overall gene expression in response to stress.