David Andreu

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Current Projects 

Synthetic peptides are useful in many areas of biomedical research, including well-known applications such as immunogens (anti-peptide antibodies, vaccines), affinity capture and purification ligands, intracellular delivery shuttles or anti-infectives. Our facility performs custom peptide synthesis and downstream operations such as purification to user-defined specifications, conjugation to carrier proteins to generate antibodies, or immobilization on affinity columns. During 2015-16 the facility has performed over 350 synthesis jobs, 55 conjugations to carrier proteins for antibody production, and related tasks for 60 users, of which 15 are intramural (PRBB) and 45 external (research institutes, biotech and pharma companies). Sizes have ranged from 5 to 60+ residues, with an average of 25. Purities up to >98% (HPLC) are routinely achieved, as well as user-defined modifications such as different end (N- and C-terminal) groups, disulfide formation (intra- or intermolecular), modified (D-amino acid, phosphorylated, acetylated, methylated) amino acids, plus biotinylation, lipidation, fluorescent tags, etc. Average turnover time for most jobs (including synthesis, HPLC purification, analytical documentation) is 1-2 weeks.

Team during 2017-18

• Technicians: Javier Valle, Yolanda Tor.