Quantitative and transcriptomic and translatomics to identify key determinants for emerging viruses infection and adaptation to vertebrate and insect hosts
Juana Diez, Universitat Pompeu Fabra, Barcelona, Spain
Nuria Busquets, IRTA, Campus de la Universitat Autònoma de Barcelona, Barcelona, Spain
Reut Shalgi, Technion, HAIFA, Israel
Sebastian Leidel, Max Planck Institute for Molecular Biomedicine, Muenster, Germany
Emerging mosquito-borne viruses such as the Chikungunya virus (CHIKV) critically rely on their capacity to multiply in mosquitoes and humans. This project will use RNAseq and ribosome profiling, a novel exciting technology that allows high-precision, quantitative measurements of transcriptome-wide translation at codon resolution, to decipher the host and CHIKV gene expression in mosquito and mammalian cells in vitro and in vivo, to identify critical nodes that determine the establishment of CHIKV infection and to identify novel therapeutic strategies. This project will define a paradigm for the analysis of other serious emerging viruses in the future.