Back Boronat, Susanna


Department of Medicine and Life Sciences (MELIS)
Teaching Assistant
  • 1983-1988: Graduate in Biology at the Universidad Autónoma de Barcelona (UAB).
  • 1991-1996 Doctoral Thesis at the CID-CSIC (Spain). Ph.D. in Biochemistry and Molecular Biology.
  • 1997-1998 Postdoctoral Fellow.  Supervisor: Hélène Richard-Foy, LBME, CNRS, UPR9006, Toulouse, France.
  • 1999-2004 Postdoctoral Fellow.  Supervisor: Judith L. Campbell, California Institute of Technology, USA.
  • 2004-2009 Postdoctoral Fellow.  Supervisor: Benjami Piña, CID-CSIC, Barcelona, Spain.


Boronat, S., Cabrera, M., Vega, M., Alcalá, J., Salas-Pino, S., Daga, R.R., Ayté, J. and Hidalgo, E. (2023) Formation of transient protein aggregate-like centers is a general strategy postponing degradation of misfolded intermediates. Int. J. Mol. Sci. 24:11202.

Marte, J., Boronat, S., Barrios, R., Barcons-Simón, A., Bolognesi, B., Cabrera, M., Ayté, J. and Hidalgo, E. (2022) Expression of Huntingtin and TDP-43 Derivatives in Fission Yeast Can Cause Both Beneficial and Toxic Effects. Int. J. Mol. Sci. 23:3950.

Boronat, S., Cabrera, M. and Hidalgo, E. (2021) Spatial sequestration of misfolded proteins as an active chaperone-mediated process during heat stress. Curr. Genet. 67:237-243.

Boronat, S., Marte, L., Vega, M., García-Santamarina, S., Cabrera, M., Ayté, J. and Hidalgo, E. (2020) The Hsp40 Mas5 connects protein quality control and the general stress response through the thermo-sensitive Pyp1.  iScience 23:101725.

Cabrera, M., Boronat, S., Marte, L., Vega, M., Pérez, P., Ayté, J. and Hidalgo, E. (2020) Chaperone-facilitated aggregation of thermo-sensitive proteins shields them from degradation during heat stress. Cell Rep. 30:2430-2443.

Marte, L., Boronat, S., García-Santamarina, S., Ayté, J., Kitamura, K. and Hidalgo, E. (2020) Identification of ubiquitin-proteasome system components affecting the degradation of the transcription factor Pap1. Redox Biol. 28:101305.

Henríquez-Olguín C., Boronat, S., Cabello-Verrugio, C., Jaimovich, E., Hidalgo, E. and Jensen, T.E. (2019) Emerging role of NADPH oxidase 2 in skeletal muscle redox signaling and metabolism. Antioxid. Redox Signal. 31:1371-1410.

Carmona, M., de Cubas, L., Bautista, E., Moral-Blanch, M., Medrano-Fernández, I., Sitia, R., Boronat, S., Ayté, J. and Hidalgo, E. (2019) Monitoring cytosolic H2O2 fluctuations arising from altered plasma membrane gradients or from mitochondrial activity. Nat. Commun. 10:4526.

Marte, L., Boronat, S., García-Santamarina, S., Ayté, J., Kitamura, K. and Hidalgo, E. (2020) Identification of ubiquitin-proteasome system components affecting the degradation of the transcription factor Pap1. Redox Biol. 28:101305.

Boronat, S., Domènech, A., Carmona, M., García-Santamarina, S., Bañó, M.C., Ayté, J. and Hidalgo, E. (2017). Lack of a peroxiredoxin suppresses the lethality of cells devoid of electron donors by channelling electrons to oxidized ribonucleotide reductase.  PLoS Genet. 13(6):e1006858.

Boronat, S., Domènech, A. and Hidalgo, E. (2017) Proteomic characterization of reversible thiol oxidations in proteomes and proteins. Antioxid. Redox Signal. 26:329-344.

Boronat, S., García-Santamarina, S. and Hidalgo, E.  (2015) Gel-free proteomic methodologies to study reversible cysteine oxidation and irreversible protein carbonyl formation.  Free Radic. Res. 49:494-510.

García-Santamarina, S., Boronat, S. and Hidalgo, E. (2014) Reversible cysteine oxidation in hydrogen peroxide sensing and signal transduction. Biochemistry 53:2560:2580.

Paulo, E., García-Santamarina, S., Calvo, I.A., Carmona, M., Boronat, S., Domènech, A., Ayté, J. and Hidalgo, E. (2014) A genetic approach to study H2O2 scavenging in fission yeast - distinct roles of peroxiredoxin and catalase.  Mol. Microbiol. 92:246-257.

García-Santamarina, S., Boronat, S., Domènech, A., Ayté, J., Molina, H. and Hidalgo, E. (2014) Monitoring in vivo reversible cysteine oxidation in proteins using ICAT and mass spectrometry. Nat. Protoc. 9:1131-1145.

Boronat, S., Domènech, A., Paulo, E., Calvo, I.A., García-Santamarina, S., García, P., Encinar del Dedo, J., Barcons, A., Serrano, E., Carmona, M. and Hidalgo, E.  (2014) Thiol-based H2O2 signalling in microbial systems.  Redox Biol. 2:395-399. 

Calvo, I.A., Boronat, S., Domènech, A., García-Santamarina, S., Ayté, J. and Hidalgo, E.  (2013)  Dissection of a redox relay: H2O2-dependent activation of the transcription factor Pap1 through the peroxidatic Tpx1-thioredoxin cycle. Cell Reports 5:1413-1424.

García-Santamarina, S., Boronat, S., Ayté, J. and Hidalgo, E. (2013) Methionine sulfoxide reductases revisited: Free methionine as a primary target of H2O2 stress in auxotrophic fission yeast. Mol. Microbiol. 90:1113-1124.

García-Santamarina, S., Boronat, S., Calvo, I.A., Rodríguez-Gabriel, M., Ayté, J., Molina, H. and Hidalgo, E. (2013)  Is oxidized thioredoxin a major trigger for cysteine oxidation? Clues from a redox proteomics approach.  Antioxid. Redox Signal. 18:1549-1556.

García-Santamarina, S., Boronat, S., Espadas, G., Ayté, J., Molina, H.  and Hidalgo, E. (2011) The oxidized thiol proteome in fission yeast - optimization of an ICAT-based method to identify H2O2-oxidized proteins.  J. Proteomics 74:2476-2486.

Boronat, S. and Campbell, J.L. (2008) Linking mitosis with S-phase: Cdc6 at play. Cell Cycle 7:597-601.

Boronat ,S. and Campbell, J.L. (2007) Mitotic Cdc6 Stabilizes Anaphase-Promoting Complex Substrates by a Partially Cdc28-Independent Mechanism, and This Stabilization Is Suppressed by Deletion of Cdc55. Mol. Cell Biol. 27:1158-1171.

Boronat, S., Casado, S., Navas, J.M., and Piña, B. (2007) Modulation of Aryl-Hydrocarbon Receptor Transcriptional Activation by Carbaryl, a non conventional ligand. FEBS J. 273:3327-3329.

Noguerol, T.N., Boronat, S., Jarque, S., Barceló, D. and Piña, B. (2006) Detection of hormone receptor ligands in yeast by fluorogenic methods. Talanta 69:351- 358.

Noguerol, T.N., Boronat, S., Casado, M., Raldua, D., Barceló, D. and Piña, B. (2006) Evaluating the interactions of vertebrate receptors with persistent pollutants and antifouling pesticides using recombinant yeast assays. Anal. Bioanal. Chem. 385:1012-1019.

Boronat, S. and Piña, B. (2006) Development of RNR3-and RAD54-GUS reporters for testing genotoxicity in Saccharomyces cerevisiae. Anal. Bioanal. Chem. 386:1625-1632.

Boronat, S, and Benjamin Piña, B. (2005) Development of yeast-based fluorogenic biosensors for detection of DNA-damaging agents in environmental samples. Yeast 22:S1- S236.

Boronat, S., Richard-Foy, H., and Piña, B. (1997) Specific deactivation of the MMTV-LTR Promoter upon Continuous Hormone Treatment. J. Biol. Chem. 272:21803-21810.