José Ayté

Studies and academic degrees:

• 1980-1985: Graduate in Pharmacy at the University of Barcelona with a grade-point average of Excellent (3).
• 1986-1990: Doctoral Thesis on "Mitochondrial and cytosolic HMG-CoA synthases are encoded by two different genes", directed by Dr. Fausto García Hegardt at the Department of Biochemistry, School of Pharmacy, University of Barcelona (Spain). Ph.D. in Biochemistry and Molecular Biology.
• 1992-1997: Postdoctoral Fellow.  Supervisor: James A. DeCaprio, Dana-Farber Cancer Institute and Harvard Medical School, Boston, USA.  The research topic was “Cell cycle control in Schizosaccharomyces pombe”.
• 1998-1999: Postdoctoral fellow.  Supervisor: Paul Nurse, Imperial Cancer Research Fund, London, UK.  The research topic was “Cell cycle control in Schizosaccharomyces pombe”.
• 1999-present: Professor at Universitat Pompeu Fabra, Barcelona, Spain.

Publications :

Sansó, M., Vargas-Pérez, I., García, P., Ayté, J. and Hidalgo, E. 2011. Nuclear roles and regulation of chromatin structure by the stress-dependent MAP kinase Sty1 of Schizosaccharomyces pombe. Mol. Microbiol. 82:542-554.

García-Santamarina, S., Boronat, S., Espadas, G., Ayté, J., Molina, H.  and Hidalgo, E. 2011. The oxidized thiol proteome in fission yeast - optimization of an ICAT-based method to identify H ₂O ₂-oxidized proteins.  J. Proteomics 74:2476-2486.

Sansó, M., Vargas-Pérez, I., Quintales, L., Antequera, F., Ayté, J. and Hidalgo, E. 2011. Gcn5 facilitates Pol II progression, rather than recruitment to nucleosome-depleted stress promoters, in Schizosaccharomyces pombe. Nucleic Acids Res. 39:6369-6379.

Ivanova, T., Gómez-Escoda, B., Hidalgo, E. and Ayte, J. 2011. G1/S transcription and the DNA-synthesis checkpoint: common regulatory mechanisms. Cell Cycle 10:912-915.

Gómez-Escoda, B., Ivanova, T., Calvo, I.A., Alves-Rodrigues, I., Hidalgo, E. and Ayte, J. 2011. Yox1 links MBF-dependent transcription to completion of DNA synthesis. EMBO Reports 12:84-89.

Zuin, A., Castellano-Esteve, D., Ayté, J and Hidalgo, E. 2010. Living on the edge: stress and activation of stress responses promote lifespan extension. Aging 2(4):231-237.

Zuin, A., Carmona, M., Morales-Ivorra, I., Gabrielli, N., Vivancos, A.P., Ayté, J. and Hidalgo, E. 2010. Life-span extension by calorie restriction relies on the Sty1 MAP kinase stress pathway. EMBO J. 29:981-991.

Calvo, I.A., Gabrielli, N., Iglesias-Baena, I., García-Santamarina, S., Hoe, K.L., Kim, D.U., Sansó, M., Zuin, A., Pérez, P., Ayté, J. and Hidalgo, E. 2009. Genome-wide screen of genes required for caffeine tolerance in fission yeast. PLoS ONE 4(8):e6619.

Moldón, A. and Ayté, J. 2009. At the (3') end, you'll turn to meiosis. Nat. Struct. Mol. Biol. 16:350-351.

Moldón, A., Malapeira, J., Gabrielli, N., Gogol, M., Gómez-Escoda, B., Ivanova, T., Seidel, C. and Ayté, J. 2008. Promoter-driven splicing regulation in fission yeast. Nature 455:997-1000.

Zuin, A., Calvo, I.A., Gabrielli, N., García-Santamaría, S., Hoe, K.-L., Kim, D.U., Park, H.-O., Hayles, J., Ayté, J. and Hidalgo, E. 2008. Mitochondrial dysfunction increases oxidative stress and decreases chronological life span in fission yeast. PLoS ONE 3:e2842.

Sansó, M., Gogol, M., Ayté, J., Seidel, C. and Hidalgo, E. 2008. The transcription factors Pcr1 and Atf1 have distinct roles in stress- and Sty1-dependent gene regulation. Eukaryot. Cell 7:826-835.

Malapeira, J., Moldón, A., Hidalgo, E., Smith, G.R., Nurse, P. and Ayté, J. (2005) A meiosis-specific cyclin regulated by splicing is required for proper progression through meiosis.  Mol. Cell Biol. 25: 6330-6337.

Zuin, A., Vivancos, A.P., Sansó, M., Takatsume, Y., Ayté, J., Inoue, Y. and Hidalgo, E. (2005) The glycolytic metabolite methylglyoxal activates Pap1 and Sty1 stress responses in Schizosaccharomyces pombe.  J. Biol.. Chem  280:36708-13

Vivancos, A.P., Castillo, E.A., Biteau, B., Nicot, C., Ayté, J., Toledano, M.B. and Hidalgo, E. (2005)  A cysteine-sulfinic acid in peroxiredoxin regulates H2O2-sensing by the antioxidant Pap1 pathway. Proc. Natl. Acad. Sci. USA. 102:8875-8880

Vivancos, A.P., Castillo, E.A., Jones, N., Ayté, J. and Hidalgo, E. (2004) Activation of the redox sensor Pap1 by hydrogen peroxide requires modulation of the intracellular oxidant concentration. Mol. Microbiol.  52: 1427-35 

Castillo, E.A., Vivancos, A.P., Jones, N., Ayté, J. and Hidalgo, E. (2003) Schizosaccharomyces pombe cells lacking the Ran-binding protein Hba1 show a multidrug resistance phenotype due to constitutive nuclear accumulation of Pap1. J. Biol. Chem. 278:40565-72

*Castillo, E.A, *Ayté, J., Moldón, A., Jones, N. and Hidalgo, E. (2002) Hydrogen peroxide and diethylmaleate activate the transcription factor Pap1 by independent redox mechanisms. Mol. Microbiol. 45: 243-54  *Co-authors

Borgne, A., Murakami, H., Ayté, J. and Nurse, P. (2002) The G1/S cyclin Cig2p during meiosis in fission yeast.  Mol. Biol. Cell 13:2080-2090

Ayté, J., Schweitzer, C., Zarzov, P., Nurse, P. and DeCaprio, J.A. (2001) Feedback regulation of  the MBF transcription factor by ciclin cig2.  Nature Cell Biol.  3:1043-1050.

Ayté, J., Leis, J. and DeCaprio, J.A. (1997)  The fission yeast protein p73res2 is an essential component of the mitotic MBF complex and a regulator of meiosis. Mol. Biol. Cell 17: 6246-54

Ayté, J., Leis, J., Herrera, A., Tang, E., Yang, H. and DeCaprio, J.A. (1995)  The Schizosaccharomyces pombe MBF complex requires heterodimerization for entry into S phase. Mol. Cell. Biol. 15: 2589-2599

Royo, T., Pedragosa, M.J., Ayté, J., Gil-Gómez, G., Vilaró, S. and Hegardt, F.G. (1995) Immunolocalization of mitochondrial 3-hydroxy-3-methylglutaryl CoA synthase in rat liver. J. Cell Physiol. 162: 103-109.

Asins, G., Rosa, J.L., Serra, D., Gil-Gómez, G., Ayté, J., Bartons, R., Tauler, A. and Hegardt, F.G. (1994) Gene expression of enzymes regulating ketogenesis and fatty acid metabolism in regenerating rat liver. Biochem. J. 299:65-69.

Royo, T., Pedragosa, M.J., Ayté, J., Gil-Gómez, G., Vilaró, S. and Hegardt, F.G. (1993) Testis and ovary express the gene for the ketogenic mitochondrial-3-hydroxy-3-methylglutaryl-CoA synthase. J. Lipid Res. 34: 867-874.

Ayté, J., Gil-Gómez, G. and Hegardt, F.G. (1993) Methylation pattern of the mitochodrial 3-hydroxy-3-methylglutaryl coenzyme A synthase promoter and its 5' flanking region during development. Biochem. J. 295: 807-812.

Gil-Gómez, G., Ayté, J. and Hegardt, F.G. (1993) The rat mitochondrial 3-hydroxy-3-methylglutaryl coenzyme A synthase gene contains elements that mediate its multihormonal regulation and tissue-specificity. Eur. J. Biochem. 213: 773-779.

Ayté, J., Gil-Gómez, G. and Hegardt, F.G. (1993) Structural characterization of the 3' non-coding region of the gene encoding rat mitochondrial 3-hydroxy-3- methylglutaryl Coenzyme A synthase. Gene 123: 267-270.

Casals, N., Roca, N., Guerrero, M., Gil-Gómez, G., Ayté, J. Ciudad, C.J. and Hegardt, F.G. (1992) Regulation of the expresión of the mitochondrial HMG-CoA synthase gene. Biochem. J. 283: 261-264.

Royo, T., Ayté, J., Albericio, F., Giralt, E., Haro, D. and Hegardt, F.G. (1991) Diurnal rhythm of rat liver cytosolic 3-hydroxy-3-methylglutaryl-CoA synthase. Biochem. J. 15: 61-64.

Gil-Gomez, G., Marrero, P.F., Haro, D., Ayté, J. and Hegardt, F.G. (1991) Characterization of the gene encoding the 10 kDa polypeptide of photosystem II from Arabidopsis thaliana. Plant Mol. Biol. 17: 517-522

Ayté, J., Gil-Gomez, G. and Hegardt, F.G. (1990) Nucleotide sequence of a rat liver cDNA encoding the cytosolic 3-hydroxy-3-methylglutaryl coenzyme A synthase. 18:3642

Ayté, J., Gil-Gomez, G., Haro, D., Marrero, P.F. and Hegardt, F.G. (1990) Rat mitochondrial and cytosolic 3-hydroxy-3-methylglutaryl-CoA synthases are encoded by two different genes. Proc. Natl. Acad. Sci. USA. 87:3874-3878.

Haro, D, Marrero, P.F., Ayte, J. aad Hegardt, F.G. (1990) Identification of a cholesterol-regulated 180-kDa microsomal protein in rat hepatocytes. Eur. J. Biochem. 188:123-129